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Revista Cubana de Medicina Tropical

Print version ISSN 0375-0760On-line version ISSN 1561-3054


GARCIA SANCHEZ, Hilda M.; GARCIA IMIA, Luis G.  and  TALAVERA CORONEL, Arturo. Estandarización y evaluación del ensayo vibriocida modificado. Rev Cubana Med Trop [online]. 1999, vol.51, n.3, pp.156-159. ISSN 0375-0760.

The vibriocidal antibody test is a reliable and well-documented method to determine bacterial antibodies to Vibrio cholerae 01 antigens. It consists of mixing serum dilutions and a steady quantity of bacteria and supplement to cause cell lysis. Titer is determined by visual observation. In this paper, we implemented a change in the presented method where a pH and glucose indicator was added to the culture medium used to stop the reaction, which allowed a quicker reading by any person who are not very familiar with the carrying out of this test since the colour change in the plaque is quite evident.

Keywords : ANTIBODIES, BACTERIAL [blood]; VIBRIO CHOLERAE [immunology].

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