My SciELO
Services on Demand
Article
Indicators
- Cited by SciELO
Related links
- Similars in SciELO
Share
Revista Cubana de Medicina Tropical
On-line version ISSN 1561-3054
Abstract
GARCIA SANCHEZ, Hilda M.; GARCIA IMIA, Luis G. and TALAVERA CORONEL, Arturo. Estandarización y evaluación del ensayo vibriocida modificado. Rev Cubana Med Trop [online]. 1999, vol.51, n.3, pp. 156-159. ISSN 1561-3054.
The vibriocidal antibody test is a reliable and well-documented method to determine bacterial antibodies to Vibrio cholerae 01 antigens. It consists of mixing serum dilutions and a steady quantity of bacteria and supplement to cause cell lysis. Titer is determined by visual observation. In this paper, we implemented a change in the presented method where a pH and glucose indicator was added to the culture medium used to stop the reaction, which allowed a quicker reading by any person who are not very familiar with the carrying out of this test since the colour change in the plaque is quite evident.
Keywords : ANTIBODIES, BACTERIAL [blood]; VIBRIO CHOLERAE [immunology].