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Revista Cubana de Medicina Tropical

Print version ISSN 0375-0760On-line version ISSN 1561-3054


VILASECA MENDEZ, Juan C.; TAMARGO MARTINEZ, Isis  and  PEREZ MONRRAS, Miriam. Producción y purificación parcial de la hemolisina principal (Neumolisina) de Streptococcus pneumoniae. Rev Cubana Med Trop [online]. 1999, vol.51, n.3, pp.160-165. ISSN 0375-0760.

The capacity of 36 Cuban strains of Streptococcus pneumoniae to produce pneumolysin was studied, and 94.4 % of them were determined to be producers of that enzime. One of the best producers was cultured at a great scale and the pneumolysin found in the supernatan was partially purified through an ion-exchange chromatography in mono-Q column. This method made it possible to recover the enzime whose purity level increased by 4.39 with 100 % output.

Keywords : HEMOLYSINS [isolation and purification]; STREPTOCOCCUS PNEUMONIAE [metabolism].

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