Revista Cubana de Medicina Tropical
On-line version ISSN 1561-3054
MAS LAGO, Pedro et al. Valoración de una novedosa tecnología para la detección de enterovirus en aguas negras. Rev Cubana Med Trop [online]. 1999, vol.51, n.3, pp. 166-171. ISSN 1561-3054.
This paper reported the appraisal of a novel thechnology for the detection of enterovirus in sewage based on a viral recovery method using polyethilenglycol as a concentrating agent and on the combined use of viral isolation and polymerase chain reaction as viral detection and identification techniques. It was also confirmed that the viral recovery method is highly efficient since it allows to recover all viruses present in sewage and to preserve their infective capacity. It was proved that the polymerase chain reaction (PCR) is less sensitive than cell culture for detecting enteroviruses in sewage. It is also possible to use guadinine isothiocyanate, whose components can be prepared in the lab, as an alternative method for taking out and purifying nucleic acids instead of using the conventional TRIZOL method wich is the one recommended in these cases by the World Health Organization.
Keywords : SEWAGE [virology]; ENTEROVIRUS [isolation and purification]; POLYMERASE CHAIN REACTION [methods].