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Revista Cubana de Medicina Tropical

Print version ISSN 0375-0760On-line version ISSN 1561-3054


MENDIOLA MARTINEZ, Judith; HERNANDEZ ALVAREZ, Hilda  and  FINLAY VILLALVILLA, Carlos M.. Actividad semejante a catepsina L en la hemolinfa de hembras repletas de Boophilus microplus. Rev Cubana Med Trop [online]. 1999, vol.51, n.3, pp.201-203. ISSN 0375-0760.

The cysteine-proteinase activity present in the hemolymph of females crammed with Boophilus microplus at the second oviposition was proved. The enzime assay was performed using the synthetic N-carbobenzoxi-phenilalanyl-arginil-4-metoxi-betan-naphthylamide substrate, and hydrolisis in both raw ovarim extract and hemolymph were observed. The activity was 100 % inhibited by trans-epoxisuscinyl-L- leucidamide (4-guadinine) butane, an specific inhibitor for cysteine-proteinase. Polyacrylamide and gelatin-gel electrophoresis showed the existance of only one active band in both samples tested with the same electrophoretic sliding (approximately 38 kDa).

Keywords : CATHEPSINS [blood]; HEMOLYMPH.

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