Abstract

FALCON MARQUEZ, Rosabel; TORANO PERAZA, Gilda; MARTINEZ, Berta Victoria  and  TAMARGO MARTINEZ, Isis. Novedoso esquema de inmunización para la obtención de anticuerpos anti-LPS de Haemophilus influenzae tipo B. Rev Cubana Med Trop [online]. 2001, vol.53, n.1, pp.67-68. ISSN 0375-0760.

A novel immunization schedule was tested to increase antibodies to Haemophilus influenzae type B in which a 0.5 % formaldehyde-inactivated cellular antigen and a partially purified lipopolysacharid antigen obtained from a selective precipitation with organic solvents were combined as immunogens. High specificity and biological activity antibodies with titers up to 1:256 were obtained by counterinmunoelectrophoresis; they were purified through a selective precipitation with ammonium sulphate and an ion-exchange chromatography using a DEAE-Sapharose column. The method proposed in this paper facilitates the production of purified antibodies useful for the improvement of serological diagnosis and H. influenzae b strain serotyping techniques.

Keywords : HAEMOPHILUS INFLUENZAE TYPE B; IMMUNIZATION SCHEDULE; COUNTERIMMUNO-ELECTROPHORESIS [methods]; LIPOPOLYSACCHA-RIDES.X.

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