Abstract

MONTALVO ALVAREZ, Ana Margarita et al. Construcción de una biblioteca genómica de Leishmania amazonensis y su expresión en músculo de ratones BALB/c. Rev Cubana Med Trop [online]. 2001, vol.53, n.3, pp. 154-160. ISSN 1561-3054.

A genomic library of Leishaminia amazonensis was built through a pcDNA3 vector, with expression promoter in eukaryot cells, to contribute to the application of immunization technology with nucleic acids in leishmaniasis. To show the expression genomic library in the muscles of mice immunized with it, the indirect immunofluoresce technique was used. A mix of sera with high antileishmania titers from an area where L.braziliensis infection is predominant was used as primary antibody. A library of 80% recombinant clones was obtained. Antigen determinant expression was confirmed in immunized BALB/c mice´s muscles, according to the results of immunofluorescence testing.

Keywords : IMMUNIZATION [methods]; NUCLEIC ACIDS [immunology]; GENOMIC LIBRARY; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; ANIMALS, LABORATORY; MICE, INBRED BALBC [immunology]; LEISHMANIA MEXICANA; LEISHMANIASIS [immunology].

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