Revista Cubana de Medicina Tropical
On-line version ISSN 1561-3054
DELGADO, Iselys; VAZQUEZ, Susana; BRAVO, José Ramón and GUZMAN, María Guadalupe. Predicción del serotipo del virus del dengue mediante la respuesta de anticuerpos IgM. Rev Cubana Med Trop [online]. 2002, vol.54, n.2, pp. 113-117. ISSN 1561-3054.
A study of 4 groups of serum samples from Cuba, Costa Rica, Nicaragua and Panama was made to determine the serotypes of dengue virus causing epidemics or outbreaks by using IgM antibody-capture ELISA(MAC-ELISA). Dengue-IgM diagnostic kit was independently used in each of the serotypes and an index value was calculated (optical density of the sample/cut-off value) in each case, with which two statistical analyses were made: Fisher´s variances and Litter significant difference (LSD) calculation. Such calculations allowed determining that in Cuba and Panama, serotype 2 circulated whereas in Costa Rica, serotype 1 prevailed. These were the isolated serotypes in various epidemics. Regarding Nicaragua, very heterogeneous responses were obtained, the responsible serotype could not be determined since more than two serotypes co-circulated in this country.
Keywords : DENGUE VIRUS [diagnosis]; ENZYME-LINKED IMMUNOSORBENT ASSAY; SEROLOGIC TESTS.