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Revista Cubana de Medicina Tropical
On-line version ISSN 1561-3054
Abstract
VITALE, Maria et al. Comparison of different DNA extraction and polymerase chain reaction methods to detect Leptospira spp. on field samples. Rev Cubana Med Trop [online]. 2005, vol.57, n.1, pp. 51-52. ISSN 1561-3054.
It was carried out a comparison on two reference Leptospira strains DNA between different extraction methods and two polymerase chain reaction protocol. The DNA was quantified and serial dilutions were tested by polymerase chain reaction. The results showed difference in terms of recovery and sensitivity between this methods.
Keywords : Leptospira; DNA; extraction; PCR.