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Revista Cubana de Medicina Tropical

Print version ISSN 0375-0760


VALDES HERNANDEZ, Iliana; MONTORO CARDOSO, Ernesto  and  HERNANDEZ-PANDO, Rogelio. In vitro interferon gamma production in mice vaccinated with ´Mycobacterium habana´ against Mycobacterium tuberculosis antigens. Rev Cubana Med Trop [online]. 2012, vol.64, n.3, pp.279-289. ISSN 0375-0760.

Introduction: development of new antituberculosis vaccines requires the characterization of the cell-mediated immune responses induced by mycobacterial antigens. Objective: to determine the immunogenic potential of ´Mycobacterium habana´ TMC-5135 when using subcutaneous vaccine in Balb/c mice. Methods: in this study, Balb/c mice were inoculated subcutaneously with live ´Mycobacterium habana´ TMC-5135. The production of IFN gamma in cell suspensions obtained from the lungs, the spleen and the lymph nodes after stimulation with mycobacterial antigens Ag85b or culture filtrate antigens (CFA) was recorded. Results: the production of IFN gamma after stimulation with CFA and Ag85b was higher in mice vaccinated with ´M. habana´ than in animals immunized with BCG. Conclusions: these results encourage new research on ´M. habana´ as vaccinal candidate against tuberculosis.

Keywords : tuberculosis; IFN gamma; ´Mycobacterium habana´; vaccine.

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