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Revista Cubana de Medicina Tropical

Print version ISSN 0375-0760

Abstract

GOZA VALDES, Roxana  and  DIAZ RODRIGUEZ, Raúl. Genotyping of Mycobacterium tuberculosis isolates in Havana, 2009. Rev Cubana Med Trop [online]. 2014, vol.66, n.2, pp.263-272. ISSN 0375-0760.

Introduction: the search for alternatives to restriction fragment length polymorphism (RFLP) with the IS6110 probe for the genotyping of Mycobacterium tuberculosis has paved the way for the development of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing (MIRU-VNTR). Objective: evaluate the spread of M. tuberculosis genotypes in Havana in 2009. Methods: eighty isolates obtained from healthcare centers during 2009 were examined and characterized by 15-loci MIRU-VNTR typing. The genotypes were expressed as numerical codes according to the copy number of each amplified MIRU-VNTR, and they were analyzed with the online bioinformatic tool MIRU-VNTR plus. 24-loci MIRU-VNTR was used for secondary typing of isolates grouped by 15-loci MIRU-VNTR. Results: forty-one different genotypes were defined with 15-loci MIRU-VNTR. Of these, 33 were single (41.25 %), whereas 8 clustered 47 isolates (58.75%). Only 5 % of the latter could be differentiated by 24-loci MIRU-VNTR, lowering the percentage of clustering to 53.75 %. Conclusions: the high clustering values revealed by the study suggest that transmission was recent. This may have had an influence on the incidence of tuberculosis in Havana in 2009.

Keywords : Mycobacterium tuberculosis; tuberculosis; genotyping; MIRU-VNTR; molecular typing; genetic characterization; Havana.

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