SciELO - Scientific Electronic Library Online

 
vol.70 issue2Pedro Kourí Tropical Medicine Institute author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO

Share


Revista Cubana de Medicina Tropical

Print version ISSN 0375-0760On-line version ISSN 1561-3054

Abstract

RAMOS GRANJA, Rachel; ALFONSO, Yenisey; COX, Raymundo  and  MONZOTE FIDALGO, Lianet. In vitro activity of antiretroviral tipranavir against Toxoplasma gondii tachyzoites. Rev Cubana Med Trop [online]. 2018, vol.70, n.2, pp.1-8. ISSN 0375-0760.

The introduction of highly efficient antiretroviral therapy has brought about a decrease in the frequency of neurotoxoplasmosis among people with HIV. It was demonstrated experimentally that protease inhibitor antiretrovirals may also have a direct action against the parasite, in this case Toxoplasma gondii. The purpose of the study was to evaluate the antitoxoplasma activity in vitro of tipranavir, a third-generation protease inhibitor antiretroviral. To achieve this aim, determination was made of the growth inhibition caused by tipranavir in Toxoplasma gondii intracellular tachyzoites, as well as its cytotoxicity against macrophages living in the peritoneum of OF-1 mice. Additionally, evaluation was conducted of atazanavir, sulfadiazine and pyrimethamine as reference drugs. Tipranavir displayed inhibitory activity against T. gondii tachyzoites, with a IC50 of 21.2 ± 3,0 µM, similar (p> 0.05) to the one obtained with sulfadiazine (IC50= 23.3 ± 3.6 µM) and higher (p< 0.05) than atazanavir (IC50= 2.8 ± 0.7 µM) and pyrimethamine (IC50= 1.2 ± 0.2 µM). However, its CC50 value (105.9 ± 10.0 µM) was higher (p< 0.05) than that of the reference drugs atazanavir (CC50= 25.0 ± 0.5 µM), sulfadiazine (CC50= 25.2 ± 3.2 µM) and pyrimethamine (CC50= 4.4 ± 1.2 µM). This is the first time a description is provided of the in vitro activity of tipranavir against T. gondii tachyzoites.

Keywords : tipranavir; tachyzoites; Toxoplasma gondii.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License