Abstract

CAYADO GUTIERREZ, Niubys et al. Study of the molecular rearrangement of TEL/AML 1 genes in acute lymphocytic leukemia: Preliminary results. Rev Cubana Hematol Inmunol Hemoter [online]. 2000, vol.16, n.3, pp.206-210. ISSN 0864-0289.

Acute lymphocytic leukemia (ALL) represents approximately 80% of the pediatric leukemias.The existance of a cryptic translocation, the t (12;21) (p12;q22) in the type B ALL, which is not detected by the conventional cytogenetic techniques and involve the TEL and AML1 oncogenes, has been recently shown by molecular biology. This alteration is at present the most common in this leukemia and it is observed approximately in 25% of the cases. Some authors have stated that such translocation identifies a group of patients with a very favorable evolution and that's why it is considered as an indicator of good prognosis. The determination of the t (12;21) in the study of ALL has a prognostic importance and it also serves as a marker for the detection of the minimal residual disease. In our paper, we standardized the RT-PCR technique for the detection of the t (12;21) and we also analized samples from 20 pediatric patients with type B ALL, which at the time of the study was in the phase of initial diagnosis or on relapse. In the study, 5 of the 20 patients showed rearranged TEL/AML l genes, which accounted for 25% of the cases. This frequency agrees with what is reported in literature up to now

Keywords : LEUKEMIA, LYMPHOCYTIC, ACUTE [diagnosis]; ONCOGENES; CHILD; TRANSLOCATIONS (GENETIC).

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