Abstract

SOCARRAS FERRER, Berta B; MARSAN SUAREZ, Vianed; SANCHEZ SEGURA, Miriam  and  MACIAS ABRAHAM, Consuelo. Introduction of the alkaline phosphatase-alkaline antiphosphatase immunocytochemical method for the immunological classification of the acute lympho-and myeloproliferative syndromes. Rev Cubana Hematol Inmunol Hemoter [online]. 2001, vol.17, n.1, pp. 65-69. ISSN 1561-2996.

The cellular immunophenotyping was carried out in 30 patients with the diagnosis of acute lympho- and myeloproliferative syndromes by the alkaline phosphatase-alkaline antiphosphatase immunoenzimatic method (APAA) introduced in our laboratory. The CD3, CD5, CD7, CD10; CDl3, CDl5, CD22, CD33 and CD41 markers were studied by using the corresponding monoclonal antibodies, according to each case. Of the acute leukemias, 16 were lymphoid leukemias (ALL) (53.3 %) and 12 were myeloid leukemias (AML) (40 %). Among the ALL, 50 % were phenotype B and of the rest, 1 case was type T (ALL-T) (3.33 %). A patient was diagnosed acute hybrid leukemia (AHL) (3.33 %) and the other was classified as acute undifferentiated leukemia (AUL) (3.33 %). It is concluded that the APAA is faster and as efficient as other enzimatic methods for the immunologic classification of the lympho- and myeloproliferative syndromes

Keywords : LYMPHOPROLIFERATIVE DISORDERS; DISORDERS; IMMUNOENZYME TECHNIQUES [methods]; LEUKEMIA.

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