Abstract

MORERA BARRIOS, Luz M et al. Study of compatibility by serological(HLA)and cellular methods (MLC) in 22 years of work in the Hematology and Immunology Institute. Rev Cubana Hematol Inmunol Hemoter [online]. 2002, vol.18, n.3. ISSN 0864-0289.

The histocompatibility of loci ABC and locus D of the main histocompatibility system was studied by serological techniques of microlymphocytotoxicity in 383 patients with various hematological diseases. Lymphocyte reactivity was compared in the mixed lymphocyte culture of 39 of 145 identical individuals for HLA antigens, of whom 29 were negative MLC for 74,35%. There was not 100% correspondence between serological and cellular studies since there was no compatibility in all the cases for HLA class II antigens and in any case for minor histocompatibility antigens that influence both the results of MLC and the causes of failed bone marrow transplantation in identical individuals. This confirms the importance of Molecular Biology typing studies and of minor histocompatibility antigens

Keywords : MINOR HISTOCOMPATIBILITY ANTIGENS [analysis]; LYNPHOCYTE CULTURE TEST MIXED; MOLECULAR BIOLOGY; BONE MARROW TRANSPLANTATION; SEROLOGIC TESTS.