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Revista Cubana de Hematología, Inmunología y Hemoterapia
Print version ISSN 0864-0289On-line version ISSN 1561-2996
Abstract
MERLIN LINARES, Julio C; VILLAESCUSA BLANCO, Rinaldo; ARCE HERNANDEZ, Ada A and GUERREIRO HERNANDEZ, Ana M. Obtaining a preparation of C1 component (q,r,s) of the complement system. Rev Cubana Hematol Inmunol Hemoter [online]. 2002, vol.18, n.3. ISSN 0864-0289.
A concentrated preparation of component C1 (q,r,s) of the complement system in an inactive form was obtained by a precipitation method, free of contamination with the rest of the initial components of the classical pathway, that is, C2 and C4. This preparation remained stable at 2°C and in the functional assays, it was able to bind to complex EAC4, to activate components C2 and C4 to form convertase C3 and cause lysis of these cells. Hemolysis reaction was activated by C2 and inhibited by C1 inhibitor, which indicates the specificity of the obtained C1. This preparation can be used to attain a specific antiserum to obtain subcomponents C1q, C1r and C1s or to standardize the functional methods of study of C1, C2 and C1 inhibitor
Keywords : COMPLEMENT PATHWAY; CLASSICAL; COMPLEMENT 1; COMPLEMENT 1Q; COMPLEMENT 1R; COMPLEMENT 1S; COMPLEMENT 2; COMPLEMENT 4.