Abstract

MORERA BARRIOS, Luz M et al. Compatibility study by serological (HLA) and cellular methods (MLC) during 22 years of work at the Institute of Hematology and Immunology. Rev Cubana Hematol Inmunol Hemoter [online]. 2003, vol.19, n.2-3. ISSN 0864-0289.

Compatibility study by serological (HLA) and cellular methods (MLC) during 22 years of work at the Institute of Hematology and Immunology The histocompatibility of the loci ABC and of the locus D of the main histocompatibility system was studied by the serological techniques of microlymphocytoxicity in 383 patients with different hematological diseases. A comparison was made in 39 of the 145 identical individuals for the HLA antigens obtained from the family studies conducted at the Institute of Hematology and Immunology by using the cellular technique and the lymphocytary reactivity in the mixed lymphocyte culture (MLC). 29 MLC proved to be negative, accounting for 74.35 %. There was not a 100 % correspondance between the serological and cellular studies, since it was not compatibilized in all cases for class II HLA antigens and in no case for the minor histocompatibility antigens that influence on the results of the MLC and on the causes of the failure of bone marrow transplantation (BMT) in identical individuals. This confirms the importance of the typing studies of molecular biology and minor histocompatibility antigens

Keywords : HLA: ANTIGENS [genetics]; HISTOCOMPATIBILITY ANTIGENS; SEROLOGIC TEST; BONE MARROW TRANSPLANTATION; TRANSPLANTATION, HOMOLOGOUS.