Abstract

SANCHEZ SEGURA, Miriam et al. Immunophenotypical characterization of B-cell chronic lymphocytic  leukemia. Rev Cubana Hematol Inmunol Hemoter [online]. 2007, vol.23, n.2, pp. 0-0. ISSN 1561-2996.

The immunophenotypical characterization of bone marrow cells and peripheral blood from 115 patients with B-cell chronic lymphocytic leukemia  was performed by an immunocytochemical ultramicromethod  at the Institute of Hematology and Immmunology  during 13.5 years. The most frequently expressed antigens were:  HLA-DR (98 %), CD5 (94 %), CD19 (93 %), CD20 (90 %), CD22 (84 %) and IgS (76 %). The CD-5  antigen was not present in 7 patients. It was found a poor expression of myelomonocytic antigens, such as CD11b (2/27) (7.4 %) and CD11c (5/13) (38.4 %). It was observed a low expression of IgS and of CD22 membrane, since these antigens were only overexpressed in 12.5 % and 32 % of the sick, respectively.  There was a  predominance in the expression of kappa light chains. The phenotypic behaviour of the patients with B-cell chronic lymphocytic leukemia corresponded with what has been reported by other authors about this entity.

Keywords : B-cell chronic lymphocytic leukemia B; immunophenotyping; monoclonal antibodies.