Abstract

MARSAN-SUAREZ, Vianed et al. Validation of immunocytochemical ultramicromethod (UMICIQ) for immunophenotyping of pediatric acute lymphoid leukemia. Rev Cubana Hematol Inmunol Hemoter [online]. 2012, vol.28, n.3, pp. 282-288. ISSN 0864-0289.

The aim of this study was to compare the immunocytochemical ultramicromethod (UMICIQ) with flow cytometry (CMF) for the immunological diagnosis of pediatric acute lymphocytic leukemia (ALL). Bone marrow from 30 patients with ALL was processed and a minimal panel of monoclonal antibodies directed against antigens expressed on B cells (CD10, CD19 and CD20), T cells (CD2, CD3 and CD7), and myeloid (CD13) was used. The results of this study showed that both methods can identify the origin line of leukemia cells, the level of maturity and their classification in different immunological subtypes. Both UMICIQ and CMF showed high sensitivity and specificity. This study validated the immunological diagnosis of pediatric ALL by UMICIQ using CMF, which is an advanced, highly automated technology internationally used for immunophenotyping (IFC) cell hematological malignancies. Thus, it was demonstrated that immunocytochemical techniques remain very useful for IFC hematological malignancies and particularly in ALL.

Keywords : acute lymphoblastic leukemia; cell immunophenotyping; immunohistochemistry; flow cytometry.

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