Abstract

MACIAS-ABRAHAM, Consuelo et al. Phenotypic characterization of mesenchymal human stem cell from bone marrow and adipose tissue. preliminary results. Rev Cubana Hematol Inmunol Hemoter [online]. 2014, vol.30, n.2, pp.162-170. ISSN 0864-0289.

Introduction:mesenchymal stem cells (MSCs) have phenotypic and functional characteristics whichgives them a broad therapeutic potential for possible use in regenerative cell therapy, allogeneic transplant rejection and chronic inflammatory diseases. Objective: to evaluate the expression of moleculemembranes expression to identify molecular patterns characteristic of human MSCs maintained in culture. Methods:the phenotypic expression of mononuclear cells from bone marrow wereobtained by bone marrow aspiration, separated by Ficoll and cultured ex vivo between passages or subcultures 3 and 16 and adipocytes cultured obtained from enzyme extraction of adipose tissue of healthy donor. Double staining was performed for molecules CD34/CD45, CD34/CD90, CD34/CD117 and CD34/CD44. Results:preliminary results showed that cultured mononuclear cells from bone marrow between passage 4 and 8 of culture expressed 45,13 % CD34-/CD45- cells (double-negative), corresponding to 25,24 % CD34-/CD90+ cells and 96,90 % of CD34-/CD117-. Adipocytes from culture cells showed 52,3 % CD34-/CD45- (double-negative), 12,31 % cells CD34-/CD90+, 43,31 % CD34-/CD117- (double-negative). Our results suggest that both cultures were differentiated to MSCs. Adipocytes from MSCs showed 64,68 % of cells with expression of CD44 adhesion molecule conferring functional homing properties Conclusions:these preliminary results corroborate that the experimental methods used in cultivation are effective for obtaining MSCs with therapeutic purposes.

Keywords : human mesenchymal stem cells; phenotypic characterization; regenerative medicine.

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