Abstract

SANCHEZ ALVAREZ, Rosa et al. Caracterización de indicadores bioquímicos de estrés oxidativo en pacientes quemados muy graves. Rev Cubana Invest Bioméd [online]. 2000, vol.19, n.3, pp. 164-167. ISSN 1561-3011.

The behaviour of some indicators of systemic oxidative stress was characterized in 33 very critical burned patients. Samples of venous blood were taken during 4 experimental points starting from their admission. The concentrations of malondialdehyde, the products of protein oxidation, vitamin A and b-carotenes, as well as the superoxide dismutase, catalase, phospholipase A₂ (PLA₂) and elastase activity and the percentage of tripsyn inhibition were quantified. The concentrations of malondialdehyde were higher in the burned patients than in the controls all the time. Likewise, statistically significant differences were observed between the dead patients and the survivors. The values of this indicator were always higher in the first. Proteins, which are also targets of the oxidative damage, were generally elevated in burned patients compared with the controls. The activity of the FLA₂ enzymes and elastase was greater in burned patients than in the apparently sound. On the contrary, the superoxide dismutase had a higher activity in the latter. No significant differences were observed among the burned patients who had not died on the 3rd and 14th day. The catalase activity during the study was higher among the patients who died than in the control group and in those who survived the thermal aggression. The latter were not significantly different from the control group. The values of vitamin A were higher in burned patients, whereas the concentrations of b-carotenes were lower. The percentage of inhibition of tripsyn was higher among those patients that did not die compared with the dead and with the individuals from the control group. This increase was remarkable on the 3rd and 7th day.The results allowed to conclude that the indicators of oxidative damage are higher in the very severe burned patient and that the biochemical indicators of antioxidant defense change according to the time of evolution

Keywords : INDICATORS AND REAGENTS; OXIDATIVE STRESS; BURNS [enzimology]; BURNS [drug therapy]; LIPID PEROXIDATION; PHOSPHOLIPASES A [analysis]; SUPEROXIDE DISMUTASE [analysis]; CATALASE [analysis]; PANCREATOPEPTIDASE [analysis]; ALPHA 1 ANTITRYPSIN [analysis]; ANTIOXIDANTS [therapeutic use]; MALONDIALDEHYDE [analysis].

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