Revista Cubana de Investigaciones Biomédicas
On-line version ISSN 1561-3011
LAMADRID BOADA, Ana Ilsa; ROMERO AGUILERA, Ivonne; GONZALEZ MESA, Jorge Ernesto and MANDINA CARDOSO, Tania. Biomonitoring of workers exposed to pesticides. Rev Cubana Invest Bioméd [online]. 2011, vol.30, n.2, pp. 235-244. ISSN 1561-3011.
A biomonitoring of workers of the pesticide formulation plant from Managua municipality to know if the manipulation of pesticides described as potential chemical mutagens, had a genotoxic effect was carried out. The primary damage of DNA was studied by means of the comet assay and the chromosomal damage by chromosomal aberrations in lymphocytes and micronuclei in exfoliated cells of oral mucosa. For data analysis the DNA percentage in tail, the percentage of cells with chromosomal aberration and the percentage of cells with micronuclei were used. The frequency of chromosomal aberrations and of cells with micronuclei, both, in the exposed group and in the control one are within the interval reported for samples of Cuban population. The values of DNA percentage in tail, analyzed by comet assay in exposed group not showed significant differences before and after working day. The results analyzed demonstrate that manipulation of pesticides by this group of workers has not provoked any genetic damage perceptible by methods used.
Keywords : Biomonitoring; pesticides; chromosomal aberrations; micronuclei; comet assay.