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Pastos y Forrajes

Print version ISSN 0864-0394On-line version ISSN 2078-8452


LEZCANO, J. C; ALONSO, O  and  NAVARRO, Marlen. Fungal population associated to the germination process of stored seeds of Leucaena leucocephala cv. Peru. Pastos y Forrajes [online]. 2015, vol.38, n.3, pp.164-170. ISSN 0864-0394.

The objective of this research was to identify the fungi associated to stored seeds of Leucaena leucocephala cv. Peru, during the germination test. For such purpose a mycological analysis was performed on seeds with 6 and 18 months of storage (400 in each case, in four replications of 100), from the storehouse of La Rioja seed farm _belonging to the Livestock Production Enterprise Martí (Matanzas province, Cuba)_. The fungal agents were identified with the aid of taxonomic keys, and the data related to the number of germinated seeds, seeds with disease symptoms and seeds rotten by fungi were processed through the system of comparison of proportions. Eleven fungal species were diagnosed: Penicillium expansum; Aspergillus flavus,isolates 2, 3 and 4; Aspergillus niger; Fusarium oxysporum; Trichoderma sp.; Pestalotia sp.; Rhizopus stolonifer; Cladosporium sphaerospermum and Aspergillus wentii. Among them the first one stood out, with a maximum of 11,25 % of infection in the rotten 18-month seeds. The main symptoms were: seed discoloration, spots on the cotyledons and necrosis of the radicle. It is concluded that the fungal species found belong to seven of the most representative genera, which come from the field and the storehouse. The fungal agent P. expansum showed the highest infection percentage in the germinated and rotten seeds. Only six of the 11 species of fungi caused the main detected symptoms (P. expansum, A. flavus isolate 2, A. niger, F. oxysporum, Trichoderma sp. and Pestalotia sp

Keywords : Forage legumes; fungi; seed storage.

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