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Pastos y Forrajes

On-line version ISSN 2078-8452

Abstract

MATOS-TRUJILLO, Madyu et al. Expression of the peroxidase enzyme in hybrid Saccharum sp. plants inoculated with Xanthomonas albilineans Ashby (Dowson). Pastos y Forrajes [online]. 2017, vol.40, n.3, pp. 195-200. ISSN 2078-8452.

In this study the expression of the peroxidase enzyme (POX) in two sugarcane cultivars (hybrid Saccharum sp.) and the correlation with the response of each one to the invasion of the pathogen Xanthomonas albilineans, its causative agent, were evaluated. The evaluated cultivars were Mayarí 55-14 (tolerant) and Cuba 85-102 (susceptible); they were both inoculated at five months of age with the bacteria, and the controls, with sodium phosphate buffer. A completely randomized design, with three replications was used. Variance analysis (ANOVA) was performed on the data, through the statistical package Statgraphics plus version 5.1. In both cultivars the POX specific activity increased after the inoculation with the bacteria; in My55-14 it was higher with regards to C85-102 since two hours. The response to the pathogen infection was detected faster in the tolerant cultivar (2 hours) than in the susceptible one (4 hours). It is concluded that the peroxidase activity is involved in the defense processes of sugarcane against the bacteria X. albilineans; hence it can be used, because of its importance, in the identification of resistant genotypes to this pathogen, in the framework of the breeding programs of this plant, in order to guarantee obtaining higher quality biomass, for the sugar industry as well as for animal feeding.

Keywords : enzymatic activity; pathogen organisms; resistance to disease.

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