Objective

To determine the potentialities of Bacillus spp. isolates from the bioproduct IHPLUS®, as plant growth promoters and with industrial purposes.

Materials and Methods

For the inoculation, 10 mL of the product were inoculated in nutrient broth medium. Afterwards, through thermal shock at 80 ºC during 12 minutes, the vegetative forms were eliminated. Seriated dilutions were carried out, and then planting was performed in nutrient agar medium. The isolates were purified and it was verified that they corresponded to Gram-positive, endospore-producer, bacillary forms. The production of indoleacetic acid and the capacity of the isolates to solubilize tricalcium phosphate were evaluated. The chitinolytic and/or 1,3-β- glucanolytic, lipidic, proteolytic, amylolytic, cellulolytic and mannanolytic activity of such isolates.

Results

Four Bacillus spp isolates were obtained. Isolates IH7 and IH5 showed biostimulator and biofertilizer properties, by producing indoleacetic acid (23,55 μg mL-1) and solubilizing tricalcium phosphate, respectively. Isolate IH7 showed potentialities as antagonist agent of phytopathogens, because it produced hydrogen cyanide. The production of 1,3-β-glucanase and/or chitinase (95,55 %) and proteases (43,98 %) was shown. IH5 showed amylolytic and mannanolytic activity; while IH7 produced amylases and cellulases.

Conclusions

The biochemical activities shown by the obtained isolates prove the importance of the bioproduct IHPLUS® as a source for obtaining Bacillus spp. strains, with applications in different agricultural and industrial sectors.

        · abstract in Spanish     · text in Spanish     · English ( pdf ) | Spanish ( pdf )