Abstract

VALDES DE LA CRUZ, Marlyn et al. GENETIC DIVERSITY OF WILD SPECIES OF Nicotiana genus I: CHARACTERIZATION USING BIOCHEMICAL MARKERS. Rev. Protección Veg. [online]. 2010, vol.25, n.2, pp. 88-97. ISSN 2224-4697.

The characterization of wild species of the genus Nicotiana is very important for the genetic breeding programs of Nicotiana tabacum L. because they can be a source of pest resistance genes to increase the genetic variability in the only commercial species of this genus in Cuba. The objective of this work was to characterize the genetic variability in ten species of the Nicotiana genus of the germoplasm bank in Cuba through the analysis of nine isozyme systems and total proteins. The isozymes assayed were peroxidase, polyphenol oxidase, esterase, carbonic anhidrase, superoxide dismutase, diaphorase, acid phosphatase, alkaline phosphatase and malic enzyme, as well as total proteins. Isozyme electrophoresis was performed on vertical slab polyacrylamide gels (PAGE). The total polymorphism level among species in the systems studied was 92,7%, where the most polymorphic systems were peroxidase, acid phosphatase and malic enzyme with 100%. These polymorphic systems, jointly with alkaline phosphatase and total protein, were the most important markers for the identification and characterization of Nicotiana species since they allowed distinguishing band patterns for each of them. This result is a first approach to the isozyme studies in wild species from the germoplasm bank in Cuba, leading to the knowledge of the variability level of those species.

Keywords : Nicotiana; genetic diversity; polymorphism; biochemical markers.

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