Abstract

PETEIRA, Belkis; BERNAL CABRERA, A; MARTINEZ, B  and  ILEANA, Miranda. MOLECULAR CHARACTERIZATION OF Cladosporium fulvum Cooke ISOLATES FROM TOMATO IN SHELTERED CROP CONDITIONS. Rev. Protección Veg. [online]. 2011, vol.26, n.1, pp. 5-14. ISSN 2224-4697.

The leaf mold caused by Cladosporium fulvum Cooke, is a very common and destructive disease in the tomato crop. Although there are cultivars with one or more resistance genes, the fact that the fungus has a very high mutation level, results in a limited use of such materials. For that reason, the knowledge of the pathogen populations contributes to the breeding program efforts to develope strategies to extent the useful life of the materials obtained. The objective of this work was the molecular characterization of the variability in a group of C. fulvum isolates from tomato plants. The molecular characterization was carried out for 37 isolates, including identification by the 28S region encoding for RNAr amplification, PCR with specific primers directed to different genes related to pathogenicity (Avr2, Avr4, Avr4E, Avr9, Ecp1, Ecp2, Ecp4 y Ecp5) and RAPDs. All the isolates were classified as C. fulvum. Any of them amplified for the Avr9 gene, although there were high amplification percentages for the Avr2, Avr4 and Avr4E genes. The virulence of the races 2, 4, and 2.4 were more related to the presence of the Ecp2 protein (100%) than to Ecp1. All the markers used confirmed a high level of molecular variability, although no relation the geographical origin was found.

Keywords : Cladosporium fulvum; molecular characterization; Avr genes; Ecp genes; RAPDs.

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