Abstract

PETEIRA, Belkis et al. BIOCHEMICAL CHARACTERIZATION OF SIX ISOLATES OF Beauveria bassiana (BALSAMO) VUILLEMIN. Rev. Protección Veg. [online]. 2011, vol.26, n.1, pp.16-22. ISSN 1010-2752.

Beauveria bassiana (Balsamo) Vuillemin is a promisoring biological control agent for invertebrate pests. In the development of a product based on a biological control agent, the study of the mode of action and the metabolites involved and their expression by the promisoring strains is needed for selection, mass rearing, formulation and strain identity. The aim of this work was the biochemical characterization of six B. bassiana isolates by a time course experiment in different culture media. Mycelia disk of six B bassiana isolates were grown in a basal medium as the control, basal medium supplemented with chitin and basal medium supplemented with gelatine. Total proteins, chitinase and glucanase activities were assayed at 3, 5 and 7 days post inoculation. In general, isolates 33 and 9 showed the highest levels for all the parameters studied. The medium supplemented with chitin was the best for total protein and chitinase activity expression, while the media supplemented with gelatine was the best for glucanase production.

Keywords : Beauveria bassiana; enzimatic activity; total proteins; chitinases; glucanases.

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