Abstract

GONZALEZ, Ivonne et al. BIOCHEMICAL CHARACTERIZATION OF PROMISING ISOLATES OF Trichoderma spp. AS BIOLOGICAL CONTROL AGENTS. II. EXPRESSION OF GLUCANASE ACTIVITY. Rev. Protección Veg. [online]. 2011, vol.26, n.1, pp. 23-29. ISSN 2224-4697.

Trichoderma species fungi secret hydrolytic enzymes which allow them to penetrate the cell walls of phytopathogenic fungi. The glucanases are enzymes that hydrolyze the b-glucans of the cell wall of phytopathogens such as Sclerotium rolfsii, Rhizoctonia solani and Pythium sp. The aim of this work was to carry out the dynamics of glucanase induction of ten Trichoderma spp. isolates evaluating the enzymatic activities at the first, third, fifth and seventh days of the cultures in three liquid media: basal medium, basal medium supplemented with chitin 0,5% and basal medium supplemented with gelatine 0,2%. The isolates 1, 13 y 17 reached the highest levels of glucanase specific activity during the induction with the basal medium supplemented with gelatine. The basal medium also resulted to be a good inductor of those enzymes.

Keywords : Trichoderma; glucanases; biological control; biochemical characterization.

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