Abstract

QUINONES, Madelaine et al. Co-existence of potyviruses and begomoviruses in the pepper crop (Capsicum annuum L.) in Cuba. Rev. Protección Veg. [online]. 2013, vol.28, n.1, pp.36-44. ISSN 1010-2752.

The objective of this work was to determine the distribution of potyviruses and begomoviruses in areas for pepper (Capsicum annuum L.) production in Cuba. Six hundred samples were collected with viral symptoms during 2006-2010. The samples were evaluated by DAS-UMELISA method using a polyclonal antibody to PVY to detect potyvirus presence, and 50 positive plants were assessed by a RT-PCR with universal (PolyT / Poty 4) primers to confirm the result. Also, the total DNA from these plants was extracted and analyzed by non radioactive NASH method with a probe from intergenic region of TYLCV to detect the begomovirus presence; these results were confirmed evaluating 50 positive samples using a PCR with generic primers (Palv1978/PARc496). The evaluation by DAS-UMELISA showed 78, 33% of potyvirus infection from the total plants collected and the results obtained by RT-PCR allowed confirming them. A begomovirus infection of 46, 17% of was determined and the amplicons obtained by PCR corresponded in size. The mixed infections in the crop reached 39, 17%. The potyviruses and begomoviruses are present in the main producing pepper areas in Cuba, being demonstrated this in study the potyvirus prevalence in the visited areas, and a high percentage of mixed infections in this crop. This integral study will contribute to the improvement of the integrated pest management and breeding programs in the country.

Keywords : PVY; TYLCV; DAS-UMELISA; PCR; NASH.

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