Abstract

GUEVARA, Yusmila et al. In vitro effect of different concentrations of NEMACID® on eggs and juveniles of Meloidogyne incognita (Kofoid and White) Chitwood. Rev. Protección Veg. [online]. 2013, vol.28, n.1, pp. 74-76. ISSN 1010-2752.

NEMACID® was developed in Cuba for root knot nematode management. This product contains the effluents from liquid fermentation of Lecanicillium lecani (Zare and Gams) as active ingredient, and is formulated with ammonium sulfate. The objective of this test was to determine the effect of NEMACID® on eggs and juveniles (J₂) of Meloidogyne incognita (Kofoid and White) Chitwood. In the test, five concentrations of NEMACID® (18,75; 3,75; 1,875; 0,1875 y 0,01875mg.ml^-1) were evaluated using distilled water, ammonium sulfate ((NH₄)₂SO₄; 1,69mg.ml^-1) and Dazomet (1g.1000ml^-1) as controls. The CL₅₀ and CL₉₀ were calculated using Probit analysis. The evaluations were done at 24h, when the total number of non mobile juveniles and non hatched eggs from the total were counted. The result corroborated the nematicide action of the product by producing the collapse of the internal content in juveniles with an impossible recovery. NEMACID® at a concentration of 18,75 mg. ml^-1 showed a higher effect on egg hatching and juvenile mortality than Dazomet (control). The ammonium sulfate, a product accompanying the fungus in the fermentation process as part of the final formulation of NEMACID®, did not affect eggs or juveniles. Concentrations of NEMACID® equal to 3,75mg.ml^-1 or lower did not affect the eggs, with a percentage of hatching inhibition below 50%. Meanwhile, in juveniles, the concentration of 3.75mg.ml^-1 caused a mortality over 70%, without significant differences with Dazomet.

Keywords : biological control; root-knot nematodes; Lecanicillium lecanii.

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