Abstract

RODRIGUEZ FUERTE, Verónica et al. Gene overexpression in two cultivars of Solanum phureja Juz. et. Buk. in interaction with Spongospora subterranean (Wallr.) Lagerh. Rev. Protección Veg. [online]. 2014, vol.29, n.1, pp.20-32. ISSN 1010-2752.

For a better understanding of the molecular mechanisms involved in the interaction between Solanum phureja Juz. et. Buk. and Spongospora subterranean (Wallr.) Lagerh, the causal agent of powdery scab disease, a 454 transcriptome analysis was carried out using one susceptible (Criolla Colombia) and one tolerant (Criolla Latina) potato cultivars. The results revealed a marked difference in the overexpression pattern for each cultivar. In the susceptible cultivar, the response was based on the activation of genes associated with cell-wall integrity, signal transduction and stress-related genes. The most overexpressed genes were those coding for methalothionein (3297 times), phosphatase 2C (2128 times) and a pectin methylesterase inhibitor (2127 times). Overexpression in the tolerant cultivar was moderate with only two genes (a putative gene and a-Galactosidase) being transcribed more than 1000 times compared with the non-inoculated control suggesting an important constitutive defense response. Overexpression of three genes in the Criolla Latina transcriptome was confirmed by qRT-PCR.

Keywords : NGS; powdery scab; real time PCR; transcriptome.

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