Abstract

GONZALEZ- PENA FUNDORA, Dianevys; COSTALES MENENDEZ, Daimy  and  FALCON- RODRIGUEZ, Alejandro B. Effect of different culture media on Phytophthora nicotianae Breda de Haan develop. Rev. Protección Veg. [online]. 2014, vol.29, n.1, pp. 33-41. ISSN 1010-2752.

The effect of different culture media on Phytophthora nicotianae growth, sporulation, and hydrolytic enzymes secretion was studied. The liquid and solid culture media used were potato dextrose agar (PDA), vegetable juice (V8), and tobacco extract. They were combined one another and supplemented with asparagine and calcium carbonate. Phytophthora nicotianae growing on solid media containing only tobacco extract reached the plate edge in the shortest time; however, the mycelium was little branched, unlike the mycelium in the media containing V8. A greater biomass production was observed in liquid culture with V8. The zoospore number was significantly higher in the treatment combining PDA only with V8; the lowest number was obtained with tobacco extract. Polygalacturonase and xylanase activities were the highest when the liquid media contained the combination of tobacco extract and V8 juice. The culture medium V8 was shown to be useful to determine the enzymatic activity of the hydrolytic enzymes and the parameters of growth and sporulation of P. nicotianae; itreduced the time required for the trials and, consequently, the risk of contamination.

Keywords : Phytophthora nicotianae; culture media; growth; sporulation; enzymatic activity.

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