Abstract

QUINONES PANTOJA, Madelaine; CASTILLO URQUIZA, Gloria; ALFENAS ZERBINI, Poliane  and  MURILO ZERBINI, Francisco. Molecular diversity of begomoviruses in the pepper crop in Cuba. Rev. Protección Veg. [online]. 2014, vol.29, n.1, pp.57-61. ISSN 1010-2752.

The main areas for pepper production in Cuba were surveyed during 2006-2010 to determine the molecular diversity of the begomoviruses in this crop. Six hundred leaf samples with typical symptoms of viral diseases were collected, and from them, 50 samples per region were randomly selected for their evaluation. The total DNA extracted from each sample was previously tested by conventional PCR using begomovirus universal primers (Palv 1978/PARc496). The positive samples were used as a template for the full-length circular genome amplification by Rolling Circle Amplification. The amplified products were characterized by restriction fragment length polymorphism analysis (RFLP) with the enzymes Sac I, BamH I, Hind III, Cla I, Kpn I, Sal I, Pst I.Begomovirus infection was detected in 100% of the samples evaluated by conventional PCR, suggesting the presence of these viruses in all the regions of the country. The enzymes Sac I, Bamh I, Sal I and Pst I produced unique cuts in unique sites in the isolated genomes liberating a unique fragment with the expected length (2.6-2,7kb), and detecting four polymorphic profiles in these samples. The obtained results show the possible presence of different begomoviruses species circulating in the crop, the occurrence of viral recombinants of previously informed or the presence of the two viral components in these samples. These results could also be associated with the presence of the two viral components in these samples. It was made evident the molecular diversity in these species of begomoviruses in the pepper crop in Cuba.

Keywords : begomoviruses; PCR; Rolling Circle Amplification; restriction fragment length polymorphism analysis; molecular diversity.

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