Abstract

CORZO LOPEZ, Mylene et al. Detection and identification of new isolates of Xanthomonas axonopodis pv. phaseoli on common bean (Phaseolus vulgaris L.) cultivars in Mayabeque province, Cuba. Rev. Protección Veg. [online]. 2015, vol.30, n.2, pp. 97-103. ISSN 2224-4697.

ABSTRACT: With the aim of detecting and identifying Xanthomonas axonopodis pv. phaseoli, the causal agent of the disease known as common bacterial blight (CBB) on common bean, 30 leaf and pod samples were taken from naturally infected plants in different localities of Mayabeque province. The samples were examined and the causal agent isolated and identified by using the following techniques: isolation on semi-selective media (YDCA y XCP1), pathogenicity test in a susceptible cultivar (BAT 304), and the PCR technique with specific primers (p7X4c yp7X4e). Of the 30 isolates obtained, 29 caused symptoms on the cultivar used in the pathogenicity test. Different symptomatologies of the disease were shown by the artificially inoculated plants. The amplification product of the PCR was a DNA fragment with 800 bp, the size reported for the isolates of this pathogen. These results showed the presence of X. axonopodis pv. phaseoli affecting the bean growing areas of the province, a frequent problem in these areas.

Keywords : common blight; Xanthomonas axonopodis pv. phaseoli; pathogenicity.

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