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Vaccimonitor
On-line version ISSN 1025-0298
Abstract
OCHOA, Rolando et al. Guide for the standardization of enzyme immunoassays for use in vaccine trials. Vaccimonitor [online]. 2000, vol.9, n.3, pp. 13-18. ISSN 1025-0298.
The standardization of enzyme immunoassays, for use in evaluation of pre-clinical and clinical vaccine trials, was reviewed. A practical guide was made. The immunoassay principles and the assay optimization step - including the preparation of standards and controls - are analyzed. Our experience is presented.
Keywords : Standardization; enzyme immunoassays; ELISA; vaccines.