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Vaccimonitor

Print version ISSN 1025-028XOn-line version ISSN 1025-0298

Abstract

GARCIA, María de los Angeles et al. Expresión heteróloga de un péptido multiepitópico de células B de M. tuberculosis en Saccharomyces cerevisiae. Vaccimonitor [online]. 2007, vol.16, n.2, pp.15-20. ISSN 1025-028X.

Saccharomyces cerevisiae has been widely used as expression system of heterologous proteins. The aim of the present work was the expression in Saccharomyces cerevisiae of a class B multiepitopic peptide from M. tuberculosis. For this purpose, the chimerical peptide named B2 was cloned in two yeast expression vectors containing galactose and cupric sulphate regulated promoters, respectively. After induction experiments, B2 expression was analyzed by SDS/PAGE and Western Blot. By Western Blot analysis B2 expression was confirmed when induction took place overnight with 100 mM of CuSO4. No expression signal took place when galactose was used as inductor. These results show that Sacchromyces cervisiae could be a good alternative host for the expression of multiepitopic peptides from M. tuberculosis.

Keywords : Saccharomyces cerevisiae; tuberculosis; vaccine; expression; antigen.

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