On-line version ISSN 1025-0298
OLIVA, Reynaldo et al. Biomodel for evaluating attenuated Vibrio cholerae strains as human cholera vaccine candidates. I: Virulence, colonizing capacity and adherence to the intestinal mucosa. Vaccimonitor [online]. 2008, vol.17, n.1, pp. 1-6. ISSN 1025-0298.
Cholera is still a human health problem in many countries. It is an epidemic or endemic disease affecting both children and adults that causes death of untreated cases. A live oral vaccine could be the solution against this disease. In the present study a biomodel was selected and applied for the evaluation of genetically attenuated Vibrio cholerae strains as vaccine candidates. The virulence, colonizing capacity and adherence to the intestinal mucosa of the strains were evaluated using 2-4 day-old neonatal Balb/c mice, weighing from 1.5-2 g. The results obtained with this biomodel showed that genetically attenuated strains are not virulent, colonize and adhere to the intestinal mucosa. The conclusion was that the biomodel used allows the evaluation and selection of candidate strains for live oral cholera vaccines.
Keywords : biomodel; Vibrio cholerae; attenuated strains; neonatal mice.