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Vaccimonitor

Print version ISSN 1025-028XOn-line version ISSN 1025-0298

Abstract

MARTINEZ, Olga M et al. Selection of shigella sonnei strains to develop an effective shigellosis vaccine. Vaccimonitor [online]. 2008, vol.17, n.3, pp.13-20. ISSN 1025-028X.

A work methodology was developed at Finlay Institute that contributed to the selection of Shigella sonnei strains as possible vaccinal candidates against shigellosis. Strains under study , were donated by the provincial Centre of Hygiene and Epidemiology in the City of Havana and were characterized using traditional methods. The identification and of the serogroup and serotype was performed by agglutination in slides with commercial antisera. Whereas DIRAMIC 10, a semi automated equipment was used to study antimicrobial susceptibility. Results were obtained four hours after. The presence of virulence plasmid for the growth of Shigella spp. In Agar Soy Triptone with Congo Red at 0.025%, as well as outer membrane protein expression in of SDS-PAGE. In addition, animal models mice-lung and the test of Sereny were used for virulence and potency tests. Results obtained allowed the selection of the strain S. sonnei A-04 as the most adequate for the obtainment of a possible vaccinal candidate.

Keywords : Shigella sonnei; vaccinal candidate; animal models.

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