Abstract

MARTINEZ, Clara et al. Magnetically-treated CM-95 adjuvant solution compared to Freund adjuvant to obtain Coombs serum in rabbits . Vaccimonitor [online]. 2009, vol.18, n.3, pp.5-12. ISSN 1025-028X.

This paper assessed the application of the magnetically treated CM-95 solution as immunological adjuvant compared to Freund adjuvant for the obtainment of Coombs serum in rabbits. Among others, Coombs serum is very demanded in blood banks and hospitals for the clinical diagnosis of Rh conflicts and haemolytic disease of the newborn. New Zealand white rabbits were subcutaneously inoculated with human serum, obtained from 30 donors of O+ blood, together with the magnetically treated CM-95 adjuvant. The immunization schedule was of 40 days and dynamics in the titers of human anti IgG antibodies was assessed by the hemagglutination technique in tubes. The immunological quality of the antiserum was determined by the titers of heteroagglutins from A,B,O blood groups, of anti C3b¸ C3d and C4d and anti IgG, before and after purification. The Coombs serum with the magnetically treated CM-95 adjuvant solution showed values similar to those of Freund adjuvant. In addition, it meets the requirements of CECMED and FDA established for this diagnostic. These results open new perspectives to the use of the adjuvant Solution in the Coombs serum obtainment.

Keywords : Coombs serum; magnetically treated CM-95 adjuvant solution; Freund adjuvant; New Zealand white rabbits.

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