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Vaccimonitor

Print version ISSN 1025-028XOn-line version ISSN 1025-0298

Abstract

DOMINGUEZ, Aníbal et al. Cytotoxic and genotoxic evaluation of the adjuvant AFCo1 by the sperm head morphology assay in NMRI mice . Vaccimonitor [online]. 2009, vol.18, n.3, pp.13-17. ISSN 1025-028X.

This study was developed to evaluate the cytogenotoxic potential on mice spermatic cells of the candidate adjuvant, AFCol (Adjuvant Finlay Cochleate 1 Adjuvant); obtained from the proteoliposome of Neisseria meningitidis serogroup B. The AFCo1 and its diluent were administered via intranasal in a 40 µL dose (1 mg/mL), while the positive control (ciclofosfamide) and the negative one (sterile distilled water) were administered orally in 40 mg and 1 mL/kg, respectively. Rats (NMRI) were from 8 to 12 week of age with weighing from 27 to 30 g, and they were administered 5 doses with an interval of 24 hours during the first 5 days of the experiment. General toxicity (body weight), cytotoxicity of testicles (spermatic concentration) and genotoxicity (spermatic morphology) were evaluated. AFCol and its diluent provided neither general toxicity, cytotoxicity, nor genotoxicity. The cyclophosphamide produced cytotoxicity (47.77%) and genotoxicity (534.61%). It is concluded that AFCol and its diluent, could be considered non toxic for the spermatic cells with the dose level and the animal biomodel used.

Keywords : Spermatogenic cells; adjuvant; Cochleate; Neisseria meningitidis B; mice.

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