SciELO - Scientific Electronic Library Online

 
vol.21 issue3gD2 coadministered with AFCo1 by intranasal route induces protective immunity against Virus Herpes Simplex type 2 in miceValidation of the LAL method for the determination of bacterial endotoxins in injectable sodium heparin author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO

Share


Vaccimonitor

Print version ISSN 1025-028X

Abstract

TORANO, Gilda et al. Haemophilus influenzae : Characterization of the isolates recovered from invasive diseases in Cuba from 2008-2011. Vaccimonitor [online]. 2012, vol.21, n.3, pp.26-31. ISSN 1025-028X.

In order to characterize Haemophilus influenzae isolates responsible for invasive disease (ID) in Cuba during the post-vaccination period, we studied the total isolates recovered from 2008 to 2011 and submitted to the Institute «Pedro Kouri» from different provinces. Specie confirmation and serotype determination were performed by considering the factors V and X requirements and using the agglutination plate method, respectively. Biotypes were defined by testing indol, urea and ornithine. The minimum inhibitory concentration (MIC) was assessed by ellipsometer test for ampicillin, ceftriaxone, chloramphenicol and rifampicin. Twenty three isolates were identified as H. influenzae; of them, 21 were serotyped and two non-typable (HNT). Near ninety percent of the serotyped isolates were classified as serotype b and only 2 as serotype a. Nine isolates of H. influenzae b belonged to biotype I while serotype a and HNT isolates corresponded to biotype II. In one strain it was demonstrated production of ß -lactamase enzyme as well as resistance to ampicillin (MIC=8 µg/mL) and chloramphenicol (MIC=12 µg/mL). It was evident that despite the decline in the incidence of Hib ID, this serotype is still the most common in Cuba, and highlights the need for ongoing monitoring to detect vaccine failures. The antimicrobial susceptibility observed during this period might be considered as an additional benefit of vaccination.

Keywords : Haemophilus influenzae b; meningitis; vaccination.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )