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Vaccimonitor

Print version ISSN 1025-028X

Abstract

LEON-TOIRAC, Emigdio et al. Application of a human linear antibody library against the capsular polysaccharide from Neisseria meningitidis serogroup B . Vaccimonitor [online]. 2013, vol.22, n.3, pp.28-35. ISSN 1025-028X.

Neisseria meningitidis serogroup B still produces a significant morbidity and mortality. Bacterial capsular polysaccharide in serogroup B shows structural homologies with human proteins and poor immunogenicity which make difficult the development of vaccines and polyclonal and monoclonal antibodies (MAb) against it. Artificial libraries of human antibodies via the expression and selection of them in bacteriophages have become known. Those antibodies are used as specific recognizing molecules capable to join to almost any existing target, with advantages over conventional MAb due to its faster obtainment without needing animal immunization, emerging as an attractive alternative for the production of MAb against complex or particular antigens. This was a basic experimental piece of work, using a phage library that expresses variable regions of human immunoglobulins to identify ligands with the capacity to recognize N. meningitidis serogroup B polysaccharide. Polyclonal ELISA screening suggests the existence of human antibodies expressed in phages which recognized the antigen of interest.

Keywords : Neisseria meningitidis; serogroup B polysaccharide; bacteriophages; monoclonal antibodies.

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