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PEREZ, Natacha et al. Structural Characterization of Granulocyte Colony Stimulating Factor, Hebervital. Vaccimonitor [online]. 2014, vol.23, n.1, pp.1-2. ISSN 1025-028X.

Granulocyte Colony Stimulating Factor (G-CSF) is member of haemopoietic proteins group used in medication. It is obtained by recombination technique since 2000 at the Center for Genetic Engineering and Biotechnology of Cuba, and it is commercialized as Hebervital. It is recommended for patients with malignant tumors, affected by febrile neutropenia, bone marrow suppressor treatment followed by bone marrow transplant, among others. It induces disorders in the biological activity of the mature neutrophils that could increase host defense in response to pathogens like bacterial and fungal infections. The objective of this project was to perform a specific analysis to determine G-CSF purity and characterization and to demonstrate its comparability with other commercial products. The sample bands pattern in SDS-PAGE, the area percentage and the chromatographic profiles under the curves, in reverse phase high performance liquid chromatographic were similar. The enzymatic digestion and separation by proteolysis of peptides generated reproduces peptide's maps of great similarity with Neupogen.

Keywords : G-CSF; neutropenia; Hebervital; granulocyte colony stimulating factor; G-CSF; neutropenia; Hebervital.

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