Abstract

SASMAKOV, Sobirdjan A. et al. Expression of recombinant PreS2-S protein from the hepatitis B virus surface antigen in Pichia pastoris. Vaccimonitor [online]. 2021, vol.30, n.1, pp. 27-32.  Epub Feb 01, 2021. ISSN 1025-0298.

The aim of this work is the expression of the PreS2-S region of surface antigen of hepatitis B virus (HBV) in yeast Pichia pastoris. A cDNA fragment encoding the Pres2-S protein of HBV was cloned to yeast transfer vectors. Based on cloned new plasmids pPIC3.5-PreS2-S (8707 bp) and pPIC9-PreS2-S (8980 bp) the recombinant strains of P. pastoris producing the PreS2-S region of surface antigen of HBV were obtained. The PAGE electrophoresis and immunoblotting of obtained recombinant PreS2-S protein confirm the molecular weight (34 kDa) and high specificity to the HBV antibodies.

Keywords : Pichia; hepatitis B virus; recombinant DNA; recombinant proteins.

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