SciELO - Scientific Electronic Library Online

vol.26 issue3Stability of standard curves of hepatitis C virus transcripts used for viral quantificationEvaluating sterilizing filtration for API-HBsAg author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO


Biotecnología Aplicada

On-line version ISSN 1027-2852


HERNANDEZ, Luis Y et al. Modelación de la cinética de crecimiento del cultivo en perfusión de células de mamíferos. Biotecnol Apl [online]. 2009, vol.26, n.3, pp.232-236. ISSN 1027-2852.

Specific equations describing the behavior of cell growth, substrate use and product formation in stirred tank fermentors and cell bank-scale perfusion cultures (30 L working volume) were developed from basic mass balance equations. A third-order polynomial equation was statistically fitted to the restricted cell passage through the screen (0) obtained from experimentally run data to model the behavior of the culture with time, since a clear description of the hydrodynamics of the system has not yet been developed. The results of the simulation of the operation process with the VisSim software application using these equations agreed with the experimental data available. The model was used to analyze the influence of cell concentration, specific growth rate and specific product formation rate on the process during years 2000 and 2001, comparing the results obtained for both periods.

Keywords : Cultivo en perfusión; filtro rotatorio; células de mamíferos; cinética de crecimiento.

        · abstract in Spanish     · text in English     · English ( pdf )