SciELO - Scientific Electronic Library Online

vol.28 issue2Bioremediation: a tool for the management of oil pollution in marine ecosystemsDetection of conformational shifts and mutations in exon 8 from the atp7b gene in Cuban Wilson’s disease patients author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO


Biotecnología Aplicada

On-line version ISSN 1027-2852


REYTOR, Mey Ling et al. Screening of protease inhibitory activity in extracts of five Ascidian species from Cuban coasts. Biotecnol Apl [online]. 2011, vol.28, n.2, pp.77-82. ISSN 1027-2852.

One of the most promising strategies to counteract malaria is by controlling the infectivity of Plasmodium parasites with drugs aimed against their main targets. The participation of proteases (belonging to different classes) has been demonstrated in its infection mechanism, hence these proteins have become promising targets in treating this illness. This study involved the screening of crude and clarified extracts of five Cuban ascidian species for inhibitory activity of proteases belonging to different mechanistic classes. Ascidians are invertebrates that present many bioactive molecules, but, as yet, only a few protease inhibitors had been isolated from them. In this research we reported for the first time the presence of inhibitory activity of bovine pancreatic carboxypeptidase A and B, aminopeptidase N from porcine kidney and subtilisin A from Bacillus licheniformis in some ascidian extracts tested. The most promising extracts were selected in terms of percentage of inhibition and they were characterized in terms of the behaviour of the extract concentration against residual enzymatic activity; the determination of IC50 values and Specific Inhibitory Activity (SIA), and the evaluation of their proteolytic activity through zymographic assays.

Keywords : ascidians; proteolytic activity; inhibitors; malaria; proteases.

        · abstract in Spanish     · text in English     · English ( pdf )