SciELO - Scientific Electronic Library Online

vol.29 issue4Improvements to the evaluation process for diagnostic quality in laboratories using the SUMA technologyGenetic improvement of potato microtuber production in vitro by gamma irradiation author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO


Biotecnología Aplicada

On-line version ISSN 1027-2852


FERNANDEZ, Osmán; DUSTET, Julio C  and  CHICO, Ernesto. Mathematical model for the application of Metabolic Flux Analysis to CHO cells producing recombinant human erythropoietin. Biotecnol Apl [online]. 2012, vol.29, n.4, pp.246-252. ISSN 1027-2852.

Mathematical modeling of metabolism meets several important applications in the context of bioprocess engineering, such as the interpretation of cell physiology. Metabolic Flux Analysis, one of the tools of this discipline, was used in the present work to characterize the biosynthesis of recombinant human erythropoietin in CHO cells. In order to apply this method, we built a matrix of stoichiometric numbers representing the major metabolic pathways for the generation of energy and the synthesis of essential precursors for product and biomass accumulation. Equations representing the biosynthesis of recombinant human erythropoietin and the growth of CHO were also derived, conferring an advantage to the proposed model over other existing designs. The dimensions of the obtained matrix were 47 × 44, with a rank of 44 and a condition number of 83; therefore, the model has a unique solution and is not sensitive. The metabolic flux map obtained by solving the mathematical model using experimental data showed results consistent with the known biochemistry of CHO cells and with the findings of other reports on this and other mammalian cell lines. The general steps of the methodology used to obtain the proposed mathematical model are also outlined.

Keywords : metabolic flux analysis; metabolism; mathematical model; CHO; EPO.

        · abstract in Spanish     · text in English     · English ( pdf )