Abstract

LEDON, Talena; HERNANDEZ, Daneylis D; MARRERO, Karen  and  FANDO, Rafael. In vitro modeling of the environmental performance of attenuated strains of Vibrio cholerae O139. Biotecnol Apl [online]. 2014, vol.31, n.2, pp.129-135. ISSN 1027-2852.

Vibrio cholerae, serogroups O1 and O139, is the causative agent of cholera diarrheal disease. Much of the research aimed to develop oral cholera vaccines is directed to the production of live attenuated strains, such as the V. cholerae O139 TLP01 and TLP05 strains. These two strains lack CTXfprophage genes and do not produce the hemagglutinin protease, a relevant pathogenesis factor, and the mannose-sensitive hemagglutinin fimbria, which could play an important role in the environmental behavior. In this work, different in vitro models were used to study the potential environmental performance of these two strains. Their ability to produce different types of biofilms, to acquire rugose phenotype and to resist different environmental stress conditions such as the presence of chlorine, detergents or high salt concentrations, were assessed. Significantly, the TLP01 and TLP05 strains displayed characteristics that limit their in vitro survival under different stress conditions, with respect to controls and wild-type strains. Such behavior under harsh environmental settings may limit their survival and act for their containment while using them as active ingredients for the development of a cholera vaccine candidate.

Keywords : cholera vaccine; Vibrio cholerae O139; attenuated strains; environmental persistence.

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