Abstract

GONZALEZ MADARIAGA, Yisel et al. In vitro photohemolytic assessment of Cissus sicyoides L. y Achyranthes aspera. Rev Cubana Plant Med [online]. 2010, vol.15, n.3, pp.126-132. ISSN 1028-4796.

INTRODUCTION: erythrocytes are useful cells to identify potentially phototoxic agents provided by systemic administration as well as to study the phototoxicity mechanisms involving oxidative stress processes. OBJECTIVE: to evaluate the photohemolytic effect of soft extracts from aerial parts of Cissus sicyoides L. (Vitaceae) and Achyranthes aspera L. (Amaranthaceae). METHODS: an in vitro protocol using human erythrocytes as biological model; they were ultraviolet light-radiated for 90 minutes to evaluate damage in erythrocyte membranes on the basis of detected hemoglobin released into the medium. RESULTS: mild hemolysis was observed, being the photohemolytic effect lower than that of positive controls. CONCLUSIONS: the extracts from these plants were rated as non-irritating, which suggests that observed hemolysis may be caused by unstable erythrocyte membranes resulting from the existence of different metabolites in the studied extracts.

Keywords : photohemolysis; erythrocytes; in vitro; Cissus sicyoides; Achyranthes aspera.

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